author(s)
Vaishali, Davinder Singh, Punit Jhandai, Renu Gupta, Sandeep Kumar and Vinay Joshi

abstract
Mycoplasma synoviae is one of the pathogenic species causing sinusitis in chicken and turkey. Mycoplasma synoviae has also caused significant economic loses in commercial flocks of broilers. A total of 100 tissue samples (trachea, lungs and air sacs) were collected form 100 poultry flocks in different districts of Haryana. A total of 19/ 100 (19%) prevalence was found in Haryana by using 16S rRNA primers through Polymerase Chain Reaction (PCR). PCR is a simple and quick approach to screen field samples.