Artificial intelligence plays enormous role in field of disease diagnosis both human and veterinary science. In digital pathology the journey of a glass slides starts to create a virtual image. Telepathology makes this image available to view at any workstation. This saves time and increases accuracy significantly. Virtual reality (VR) is feeling the imaginary world i.e. simulation running in computer in a 3D manner with a collection of technological devices. In recent years Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI TOF MS) has emerged as a potential tool for microbial identification and diagnosis in less time.