author(s)
Raghavendra Prasad Mishra, Udit Jain, Parul, Barkha Sharma, Anita Chappalwar, Janardan Yadav and Mini Kanchan

abstract
A total of 700 bovine milk samples was taken from four districts of brij region of UP. All the samples were processed to detection of prevalence of brucellosis by MRT and ELISA and analysis risk factors associted with brucellosis. Out of 700 bovine milk sample, the prevalence of brucellosis in milk sample of cattle was found to be 05.78% (26/450) and 08.45% (38/450) positive by MRT and I- ELISA respectively. On the other hand in buffaloes milk the prevalence of brucellosis in 250 milk sample was found to be 07.20% (18/250) and 09.02% (23/250) positive by MRT and I- ELISA respectively. The seroprevalence of brucellosis to this region may have public health significane. It is recommended that good management and hygienic practices shall be performed during handling ruminants specially cattle and buffaloes.