author(s)
Yanben M Kikon, K Loganathasamy, VS Gomathy, K Vijayarani and M Vengatachalam

abstract
The experiment was conducted to study the sperm lipid peroxidation status and plasma membrane integrity of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37 ºC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25µg/mL (treatment I), 50µg/mL (treatment II) and 100µg/mL (treatment III) of HBP. The contents were incubated at 37 ºC for 2 hours. The sperm LPO status was measured by production of malondialdehyde (MDA) level. The MDA levels were significantly (P<0.05) low in HBP treatment I (1.34 µmol/ml ± 0.08) and II (1.23 µmol/ml ± 0.06) and III (1.21 µmol/ml ± 0.03) when compared to control (1.85 µMol/mL ± 0.02). MDA levels did not differ significantly among different treatments. The sperm functional membrane integrity was studied by hypo-osmotic swelling test (HOST). The functional membrane integrity of spermatozoa in HBP treatment I, II III and control were 59.20% ± 0.90, 53.50% ± 0.71,52.00% ± 0.47and 51.50% ± 0.73, respectively. Significantly (P<0.05), higher percentages of spermatozoa were lost their plasma membrane integrity in HBP treatments as compared to control. It is evident from this study that supplementation of HBP in capacitation medium reduces sperm lipid peroxidation and destabilizes plasma membrane integrity.