author(s)
Nishant Parmar, Hemant Joshi, Gajendra Singh and Balwant Meshram

abstract
The spleen of an adult Guinea fowl (Numida meleagris) bird was located at right lateral in abdominal cavity which observed to the junction between proventriculus and gizzard. Histomorphologically, the outer capsule had an unequal distribution of collagen, reticular and elastic fibers along with the few smooth muscle cells. Parenchyma were accommodated the poorly developed trabeculae with arteries and veins. Splenic parenchyma was composed of indistinctly demarcated white pulp and red pulp. The white pulp was predominantly constituted with small and medium-size lymphocytes, reticular cells and reticular fibers which was subdivided into periarterial lymphatic tissue, perivenous lymphatic tissue, periellipsoidal lymphatic tissue and ellipsoids. Germinal center was not observed in white pulp. The less capacious red pulp was evident with cords and narrow sinuses within network of reticular fibers. The sinuses were narrow and irregular spaces were lined by flattened endothelial cells. The cords were composed of lymphocytes, macrophages and granulocytes. Histochemically, the splenic capsule showed intense PAS positive activity while white pulp and red pulp showed moderate activity. Moderate acid phosphatase activity was observed in the capsule and white pulp, however the strong acid phosphatase activity was detected in red pulp. The capsule and white pulp has shown weak alkaline phosphatase activity but the red pulp has shown moderate activity of alkaline phosphatase.