author(s)
Thirunavukkarasu SB, Pushpendra Kumar, Om Prakash Amit Kumar and Ilayakumar K

abstract
MHC genes are highly polymorphic, plays a pivotal role in immune system for recognition of pathogens and parasites. The purpose of this study was to explore the polymorphism of CLA-DRB gene in Tellicherry goat using PCR-RFLP technique. A region of exon 2 encompassing 285bp fragment of DRB gene was amplified by polymerase chain reaction. The restriction enzyme digestion by TaqI revealed three genotypes AA (285/285bp), AB (285/162/123bp) and BB (162/123bp) with frequencies 0.206, 0.514 and 0.280, respectively and two alleles A and B with frequencies 0.463 and 0.537, respectively. The polymorphic information content (PIC) value and expected heterozygosity were 0.374 and 0.514, respectively. The present study showed polymorphic nature of MHC Class II DRB exon2 Gene in Tellicherry goats at this locus and the frequencies of heterozygote were greater than homozygote.