author(s)
Junmoni Boruah and Bhupen Kumar Sahu

abstract
The study was conducted to optimize different compositions of modified MS media as treatments for explant establishment, shoot initiation, shoot proliferation and root induction. Establishment of the cultures (58%) and shoot initiation rate (75%) increased significantly at P=0.05 on T₁C, MS supplemented with Kinetin (0.5mg/l), BAP (3mg/l), GA₃ (0.5mg/l) and fenbendazole (100mg/l). It was also observed that although kinetin stimulates shoot initiation and proliferation but at the same time it induced slight callusing at the base of the explants. Shoot proliferation showed best result in T₂C, MS supplemented with Kinetin (1.5mg/l), BAP (4mg/l), GA₃ (0.5mg/l) and fenbndazole (100mg/l). Development of calli was observed at the base of the shoots and failed in regenerating roots when cultured on rooting media (T₃ and T₄) Thus, it was inferred that treatment T₁C and T₂C were most effective for the establishment, shoot initiation and multiple shoot regeneration of the som explant.