author(s)
Om Prakash Dinani, Pramod Kumar Tyagi, Rokade JJ and SK Bhanja, JS Tyagi, Narayan Dutta and Gopi M

abstract
A biological experiment of 384 broiler chicks was conducted to evaluate the effect of feeding rice based distillers dried grain with solubles (rDDGS) as soybean replacement without or with different enzymes on immunity in broiler chickens for 42 days as per 3x4 factorial completely randomized design. There were twelve different treatments with 4 replicates for each treatment and each replicate consisted of 8 chicks. Two levels of rDDGS were taken (12.5 and 15%). Protease, xylanase and multienzymes supplementation under different treatments were done. The humoral immunity was significantly (P<0.01) better at 12.5% and 15% rDDGS levels compared to control. Protease enzyme supplementation improved significantly (P<0.05) humoral immunity at 15% rDDGS level. Thus, it may be concluded that 12.5% and 15% rDDGS levels with or without protease supplementation improved immune response in broiler chickens in terms of humoral immunity.