Comparative study for efficacy of Immunochromatography antigen assay (ICA) test kit with Polymerase chain reaction (PCR) to detect canine parvovirus
Jitendra Tanwar, Sunita Choudhary, Gaurav Jain and DK Bihani
Canine parvovirus infection is an acute highly contagious life-threatening disease. It affects young puppies between the times of weaning to six months of age and therefore, diagnosis is essential for screening infected puppies at early stage to prevent mortality. The objective of the present study was to compare sensitivity and specificity of immunochromatographic antigen assay (ICA) test with gold standard polymerase chain reaction. Fecal samples of one hundred dogs suspected for canine parvovirus were tested with Ubio quick VET rapid antigen test kit of which twenty two dogs (22) and in polymerase chain reaction thirty (30) dogs were found positive. Eight dogs which were detected negative with the ICA kit test were found positive for canine parvovirus with polymerase chain reaction. Relative to PCR results, sensitivity and specificity of ICA test kit was 73.33 percent and 100 percent respectively.