author(s)
Dr. Kalawati Kumari

abstract
Insecticides are the widely used chemicals in the agricultural, economic, human, and environmental sectors. Cypermethrin is a highly toxic organochlorine pesticide for aquatic species that often leads to death by impairing the metabolism. During the present study effect of cypermethrin on α-amylase activity at 24h, 48 h LC₅₀ doses was determined. Effect was also observed at sub-lethal concentration along with the determination of bio-concentration factor. Reversal of the changes along with changes in RNA content was also determined. Hepatopancreatic activity was observed and results showed that the boiled enzyme (in a water bath) showed no in-vitro enzyme activity where the enzyme activity in the ice bucket was observed as significant. It was noted, from the that the LC₅₀ doses of Cypermethrin inhibited the -amylase activity by 32.1%, 64.7% and 58.8% at 24h, 48h and 72h (LC₅₀ doses) respectively. It was found that during 24h, 48h and 72 h of exposure of fish to LC₅₀ endosulfan concentration there was 52.17%, 64.7% and 58.8% loss of enzyme activity.