author(s)
Arvınd Mohanan, Abhıshek Sharma and Mamta Pathak

abstract
The screening study was conducted to identify the germplasm resistant to begomovirus associated with bitter gourd yellow mosaic disease (BGYMD). A total of sixteen bitter gourd germplasms, procured from Punjab Agricultural University (PAU) were grown in protrays for this purpose. The virulent whiteflies fed on previously characterized infected plant source were allowed to feed on these germplasms. The per cent disease ıncidence ranged from 40 to 100 per cent. Based on 0-5 disease scale, none of the entries were found to be immune to begomovirus. However, the germplasm PAU BG-61 was found to be moderately resistant and recorded lowest per cent disease incidence. The DNA extracted from the infected plants, cross checked with Polymerase Chain Reaction (PCR) confirmed the presence of begomovirus. The molecular characterization studies revealed that the associated begomovirus was close to Tomato leaf curl New Delhi virus (ToLCNDV).