Entomopathogenic nematodes are important biocontrol agent against many insect pests. For isolation of entomopathogenic nematodes a survey was conducted. Out of one hundred cadaver of Lepidiota albistigma, ten samples were found to be positive for entomopathogenic nematodes in Sorbhog, District Barpeta, Lower Brahmaputra valley zone, Assam, India. On the basis of morphological and morphometrical studies, the isolated nematode was identified as Heterorhabditis bacteriophora.