author(s)
Basharat Maqbool Wani, Shayaib Ahmad Kamil, Showkat Ahmad Shah, Majid Shafi, Mir Shabir, Bisma Kashani, Mir Nadeem Hassan and Pankaj Goswami

abstract
The present study conducted in division of Veterinary Pathology, SKUAST Kashmir was aimed to isolate and biochemically characterize Escherichia coli from different organs of Colibacillosis affected broiler chicken. The affected live birds showed clinical signs ranging from lameness, retarded growth, head swelling, respiratory distress and ruffled feathers, whilst the dead birds were selected for the study. The dead birds on necropsy revealed lesions typical to Colibacillosis viz; Pericarditis, perihepatitis, spleenitis and peritonitis. For isolation of Escherichia coli, samples from heart, lung, liver and spleen were collected. Identification of isolates as Escherichia coli were carried out using standard cultural and biochemical tests like IMVIC. Spleen, heart and lung were the common samples found positive for Escherichia coli followed by liver. Furthermore the techniques used in this study viz; standard cultural and biochemical tests like IMVIC was found to be very efficient, accurate and certainly quicker to detect the Escherichia coli in Colibacillosis affected broiler chicken. This study was conducted in Ganderbal district of Kashmir Valley.