author(s)
Avijit Biswas, Dr. Gadadhar Dash, Prasenjit Mali, Dr. Siddhartha Narayan Joardar, Dr. T Jawahar Abraham, Sutanu Karmakar, Debapriyo Mukherjee and Dr. Koel Bhattacharya Sanyal

abstract
To develop vaccine for rohu (Labeo rohita), efficacy of three antigenic preparations from Aeromonas hydrophila were evaluated. Thirty six tanks with ten rohu were divided in quadruplicates (R₁ to R₄) with nine tanks (G₁ to G₉). Rohu of G₁ to G₆ tanks were given intraperitoneal vaccine with outer membrane protein, somatic protein and formalin-inactivated whole cell itself and along with Incomplete Freund’s Adjuvant @ 200 µg/fish, G₇ and G₈ tanks were injected with Incomplete Freund’s Adjuvant (100 µl/fish) and normal saline (100 µl/fish) respectively and G₉ tanks were kept as control. After 28 d, rohu of R₃ and R₄ were subjected to intramuscular A. hydrophila challenge (LD₅₀) @ 2.85×10⁶ cells/fish for 7 d and RPS (%) was calculated. Specific cellular and humoral immune responses were determined for rohu of R₁ and R₂. Results showed that rohu immunized with outer membrane protein along with adjuvant could offer an appropriate vaccine strategy.