author(s)
Sharma Anshu, Jumde Pratibha and Kolte SW

abstract
Tick and tick borne diseases are one of the biggest public health and veterinary problems in the world. Control of ticks using chemical acaricides have developed resistance to wide range of acaricides and also producing environmental pollution. Nowadays more emphasis is given on use of environment free biocontrol agents such as ethno veterinary herbal medicines. Use of Nicotiana tabacum (Tobacco) was a traditional control remedy for control of ectoparasites in India. In vitro acaricidal efficacy of crude aqueous leaf extract of Nicotiana tabacum on immature and adult stages of Rhipicephalus (Boophilus) microplus was carried out at concentrations of 15, 30, 45 and 60% with 12 hour time interval. The extract was found to have a significant effect on the mortality of nymphal and adult stages of ticks. The 100% mortality of Nymphal and adult stages of ticks was observed at 36 hours post treatment with 60% concentration. 100% mortality of all the Nymphal and adult stages of ticks was observed with the concentration 15, 30, 45 and 60% at 48 hours post treatment. The mortality rates increased with the extract concentrations. It is concluded that Nicotiana tabacum is effective biocontrol agent for control of ticks.