author(s)
Kayina A, Lalrintluanga K, Ahmed FA, Talukdar DJ, Kalita G, Behera P and Tolenkhomba TC

abstract
A total of 36 ejaculates were collected from three indigenous bulls (Zobawng) of Mizoram. The semen samples were evaluated to find out the effects of fructose i.e. Tris egg yolk citric acid fructose glycerol (TEYCAFG) and Egg yolk sodium citrate glycerol (EYSCG) extenders on freezability of indigenous bulls’ (Zobawng) semen of Mizoram in terms of sperm motility, per cent live sperm, HOSST reacted spermatozoa and acrosomal integrity. The mean sperm motility, per cent live sperm, HOSST and acrosomal integrity in TEYCAFG extender at pre and post freezing were (69.53±0.31 and 45.36±0.59%), (76.36±0.35 and 53.03±0.45%), (67.91±0.45 and 44.92±0.56%) and (75.17±0.58 and 63.25±0.56%) respectively, and in EYSCG extender were (45.67±0.48 and 33.08±0.36%), (54.56±0.41 and 38.08±0.28%), (47.86±0.44 and 34.61±0.40%) and (57.22±0.31 and 45.89±0.48%) respectively. The mean motility, live sperm, HOSST and acrosomal integrity in both TEYCAFG and EYSCG extenders were significantly (P<0.01) higher at pre-freezing when compared to post freezing and the mean values of the said parameters were significantly (P<0.01) higher in TEYCAFG extender than EYSCG. In conclusion, the Tris egg yolk citric acid fructose glycerol (TEYCAFG) is good for freezing of indigenous bulls’ (Zobawng) semen of Mizoram.