author(s)
Ratnamma, Harischandra Naik R, Prabhuraj A, Nagaraj M Naik and Bheemanna M

abstract
The sensitive analytical QuEChERS (quick, easy, cheap, effective, rugged and safe) method with liquid chromatography tandem mass spectrometry was developed and validated as per European Commission’s guidelines and used for the determination of acetamiprid in okra. This method was validated for linearity, limits of detection, quantification, matrix effect, specificity, trueness, repeatability and reproducibility. The LOQ of the developed method was 0.002 mg kg^-1 for acetamiprid. The recovery was 74.79-94.71% at LOQ, 5 LOQ and 10 LOQ level. The% RSD values for intraday and interday precision study were ≤ 20%. For the dissipation study acetamiprid 20% SP was applied at recommended (10 g a.i. ha^-1) and double the recommended (20 g a.i. ha^-1) dose in okra. The initial deposit of acetamiprid was 2.03 and 3.11 mg kg^-1 at recommended and double the recommended doses, respectively. The studied insecticide reached below detectable limit within 30 days after spray.