author(s)
Raghvendra Kumar and Shalini Jaswal

abstract
Global proteome profile of immature buffalo oocytes (cumulus free, zona pellucida enclosed) was done and 1662 proteins were identified (FDR <1%) from isolated crude proteins. Among these proteins characterized and uncharacterized proteins were obtained. The data (no. of proteins) obtained was compared with evidenced data of other buffalo oocytes. Among the identified proteins, oocyte germ cell potential markers and RNPs associated proteins were found. Our finding with Bioinformatics analysis suggests, enriched pathways associated with immature oocyte. Our findings provide comprehensive catalogue of proteins according to biological process, molecular function and cellular component through PANTHER analysis. For pathway analysis KEGG tool was used and abundance of protein was classified in different pathways. Here we have discussed notable proteins obtained in our profile which have crucial significance towards oocyte quality and probable marker associated properties. The proteome profile of immature oocyte crude proteins found in this study can be source for further developmental studies.