author(s)
Prakash Singh, K Shrman, RK Sharma, NS Meena, Nitesh Kumar and Kamal Kishor

abstract
The present study was planned to study the safety assessment of piperine after oral administration @ 20 mg/kg body weight in 6 healthy Sirohi goats. Blood samples were collected before administration of the piperine which served as control (day 0). After administration of piperine, blood samples were collected at day 8 and 16 from jugular vein. Serum was harvested by centrifugation of vacutainers at 4000 rpm for 10 minutes at 4ºC. On day 0, the mean value of serum aspartate amino-transferase was observed to be 116.8±3.2 IU/L, which on day 8 reaches to 130.1±16.7 IU/L and on day 16, increases upto 133.1±16.7 IU/L. On day 0, 8 and 16 of safety study, the respective mean value of alanine amino-transferase (ALT) were 42.2±1.4 IU/L, 44.0±1.7 IU/L and 41.3±3.1 IU/L, respectively. The mean value of albumin was found to be 2.5±0.1 g/dl on day 0, 2.6±0.1 g/dl on day 8 and 2.6±0.1 g/dl on day 16 of the study. In Haematological examination, the mean value of haemoglobin (Hb) on day 0, 8 and 16 were 8.0±0.1 g/dl, 8.0±0.2 g/dl and 7.6±0.2 g/dl. Packed cell volume observed on day 0 was 21.8±0.3%, whereas, same was 22.4±0.4% and 22.1±0.5% on 8 and 16 day of the study.