author(s)
Kaiho Kaisa, N Shyamsana Singh, P Mayengbam and TC Tolenkhomba

abstract
The study was conducted to study the polymorphism in bovine Toll-Like Receptor 4 gene in 50 swamp buffaloes of Manipur. Two different variants of SNP1 (A-3642C) of TLR4 gene Exon 3 viz. A and C were detected by PCR-RFLP using AluI. The frequency of C allele was predominant (0.95) among swamp buffalo population of Manipur. Among the genotypes, CC genotype was prevalent in very high frequency in the population. While the genotype AA was not observed in any of the sample evaluated. The population not conforming to equilibrium indicated exertion of sampling variation due to small population size in these buffalo population.