author(s)
Surya Kumar Maurya, Ram Sagar, Padma Nibash Panigrahi and Atul Saxena

abstract
The present study was designed to determine alteration on blood mineral profile, hormonal profile and enzymatic activity in post-partum anestrous buffalo before and after supplementation of mineral mixture and antioxidants. Thirty Post-partum anestrous buffaloes were randomly selected from different blocks of Mathura, Uttar Pradesh, India and divided in to three groups 10 animals each. Group A (n=10) animals were acted as a control without having any treatment. Group B (n=10) animals were fed with mineral mixture @ 50 g daily for 25 days (composition: dicalcium phosphate- 40%, calcium carbonate-30%, sodium chloride- 22%, ferrous sulphate- 2.5%, copper sulphate-2.05%, potassium iodide-0.05%, zinc sulphate-0.75%, cobalt chloride-0.05%, manganese chloride-0.6%, magnesium sulphate-2%). Group C (n=10) animals were fed with antioxidant (vitamin E+selenium, in the proportion of 1500 IU (1.5gram) of vitamin E and 50 mg of sodium selenate equivalent to 10 ppm of selenium) for 25 days. Blood mineral profile, hormonal profile and serum enzymatic activity were determined at day 0 and 25 days of supplementation. There was a significant increase in calcium, copper, zinc, estradiol and insulin concentrations as well as total protein, glucose and ascorbic acid activities in both treatment groups. In group A, out of 10 anestrous buffalo only 1 animal exhibited estrous after 24 days. On contrary 5 animals in group B and 6 animals in group C exhibited estrous and conceived in the first service.