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Journal of Entomology and Zoology Studies
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P-ISSN: 2349-6800, E-ISSN: 2320-7078

Journal of Entomology and Zoology Studies

2021, Vol. 9, Issue 1
Determination of prevelance and molecular characterization of extended spectrum beta-lactamase (ESBL) producing Escherichia coli in poultry from J&k, India

Indica Sharma, MA Bhat, Anil Taku, Deep Shikha, Apurva Gupta, Ufaq Aijaz, GA Badroo, Faizan Javid and HK Sharma

The study was aimed at finding the prevelance of Extended Spectrum beta-lactamase (ESBLs) producing E. coli in poultry and to characterize them with respect to blaTEM, blaCTX-M and blaSHV gene. Out of total 200 faecal samples collected, 400 presumptive ESBL producing E. coli were isolated. Of the 400 isolates, 150 (37.5%) were found to be resistant to cefotaxime and ceftazidime. Resistance to cefotaxime was observed in 60.0% and ceftazidime in 40.0% isolates and 46.6% isolates showed resistance to both. All the 150 isolates were confirmed as ESBL producers by Double disc synergy test. The overall prevelance from this study is 37.5% and is the first report of its kind in Jammu region (J&K). Further, all the ESBL producers were tested for the presence of bla gene. Of 150 ESBL isolates, 110 (73.2%) carried the blaTEM, blaCTX-M and blaSHV gene/s. Of these 110 isolates, 27.2% carried blaTEM gene alone, 48.1% carried blaCTX-M gene, 1.81% carried blaSHV only and 22.72% isolates carried both blaTEM /blaCTX-M genes.
Pages : 2108-2111 | 341 Views | 112 Downloads


Journal of Entomology and Zoology Studies Journal of Entomology and Zoology Studies
How to cite this article:
Indica Sharma, MA Bhat, Anil Taku, Deep Shikha, Apurva Gupta, Ufaq Aijaz, GA Badroo, Faizan Javid, HK Sharma. Determination of prevelance and molecular characterization of extended spectrum beta-lactamase (ESBL) producing Escherichia coli in poultry from J&k, India. J Entomol Zool Stud 2021;9(1):2108-2111.

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