author(s)
Ravi Sikrodia, Daljeet Chhabra, SS Mahor, SD Audarya, Rakhi Gangil and Rakesh Sharda

abstract
The uterine infectious problems play important role in the lower conception in dairy animals and one of the major hurdles for the profitable dairy industry. Repeat breeding due to the involvement of bacteria is an important aspect for the economic loss of the farmers in respect of increased inseminations, increased calving interval and treatment. There are number of bacteria involved in repeat breeding e.g., Streptococcus spp., Staphylococcus spp., Clostridium spp., Archanobaterium spp., fusobacterium and Escherichia coli. In the present study 22 Cervico-vaginal mucus samples from the repeat breeder crossbred cows were examined for the presence of Staphylococcus spp. The samples were also screened for the presence of other bacteria and fungi. Out of these 22 samples, 9 samples were found positive for the presence of bacteria out of which 7 samples were found positive for the Staphylococcus. All the samples were found negative for the fungi. In vitro sensitivity of these isolated Staphylococcus was also checked for the 9 different antibiotics. Out of these 9 antibiotics used, ciprofloxacin and ofloxacin were showing a maximum area of sensitivity than the other antibiotics used. The study revealed that Staphylococcus also play role in repeat breeding in cattle and found sensitive for the ciprofloxacin and ofloxacin, which can be used for the treatment.