author(s)
Ghanshyam Kumar Pandey, Shafaat Ahmad, Bhushan Kewate, Anoop Yadav, Brajendra Kumar Yadav, Sunil Zacharia, Gaurav Chaudhary, Nikunj Tyagi and Pradeep Kumar Shukla

abstract
Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici is a serious problem limiting tomato production worldwide. Biological control has emerged as one of the most promising alternatives to the chemical fungicides. For the management of disease an investigation was carried out at research laboratory of the Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj. to evaluate the efficacy of bio-agents viz. Trichoderma viride, T. harzianum, Pseudomonas fluorescens, plant extracts viz. neem oil @ 10%, and neem seed kernel extract@ 10% and Neen leaf extract @ 10%, against F. oxysporum under In vitro experiment was analysed by using CRD (Complete Randomized Design) with six treatments and three replication viz. Trichoderma viride, T. harzianum, Pseudomonas fluorescens, Neem leaf extract, neem oil, and neem seed kernel extract. Observations recorded at 24 hrs, 48 hrs, and 72 hrs. 96 hrs. 120 hrs. 144 hrs, 168 hrs. T. viride was inoculated directly in PDA poured plate in a dual culture technique which gave the best results among all the treatments (1.475) followed by Trichoderma harzianum (1.765). Whereas in case of neem products neem oil @10% was best effective (5.450) followed by neem leaf extract (6.375) followed by NSKE (6.625). It was seen that all the treatments were significant over control (8.285).