author(s)
Ghanshyam Kumar Pandey, Shafaat Ahmad, Bhushan Kewate, Anoop Yadav, Brajendra Kumar Yadav, Sunil Zacharia, Gaurav Chaudhary, Nikunj Tyagi and Pradeep Kumar Shukla

abstract
Evaluate the shelf life and prepare the formulation of the isolated Trichoderma viride, Trichoderma harizianum, and Beauveria bassiana. In vivo conditions soil inoculated with Fusarium oxysporum f. sp. ciceri was conducted and compare the efficacy of different treatments viz. Seed treatment with commercial formulation of bio-control agents and fungitoxicants in the management of Gram (chickpea) wilt. The two year pooled data of Trichoderma viride Trichoderma harzianum Beauveria bassiana At 10⁶ (D₁) and 10⁷ (D₂) dilution factor was found to be significant and data indicate consequently decline in the order of cfu/g during the year of 2015-16 and 2016-17, average of both year. In Beauveria bassiana some viable propagules seen during the experiment. So it might be decline due to lesser no of viable propagules in talc based formulation of Beauveria bassiana. Inhibitory effect of Trichoderma sp. against radial growth of growth of test fungus reported highest (42.67 mm) in control T₀ highest percentage of inhibition was found in T₃ Trichoderma harizianum, Trichoderma viride (T₁). Trichoderma viride was found to be significantly superior over Trichoderma harzianum. The data suggested that selected bio-pesticide have potential to control Chickpea wilt caused by Fusarium oxysporum f. sp. ciceri and therefore could be effectively utilized in eco-friendly management of Chickpea wilt.