author(s)
Bhubaneshwari Devi Moirangthem and Dhananjoy Chingangbam Singh

abstract
Polytene chromosomes of chironomids are big and beautiful and taxonomically important. But the differentiation and collection are rather difficult. The non-seasonal and abundant sources of polytene chromosomes are the larvae of Telmatoscopus sp. which are available in every habitats. The larvae from the drainage or sewage from locality were procured and reared in a beaker and preserved the pupae to able to collect the adults. The salivary glands from the larvae were dissected out under simple microscopes in the Carnoy’s fluid I, and stained with 2% acetocarmie. The salivary gland cells with prominent nuclei with visible polytene chromosomes were further squashed. A single nucleus contain roughly 6 (six) mostly intertwined polytene chromosomes. The six distinct polytene chromosomes with a lot of unpaired regions, puffs, Balbiani’s ring, two nucleoli and inter-bands might serve the study for systematic evaluation. The difficulties of the material are that the salivary collection and observation of small chromosomes. The centromeric regions were very prominent. In future the practical classes could be done with these larvae in order to avoid difficulties in collection of the chironomids and seasonality. One other important aspect of the materials are that there is no enough literature to compare and study will light on the evolution and structural differentiation of the chromosomes particularly from the Manipur.