author(s)
Dr. Dhenge SA, Gade NE, Mishra OP, Abinash Kumar and Vaidya MM

abstract
Graphene oxide (GO) is a carbon based nanomaterial extensively used in biosciences. In present study, comparative in vitro hemolytic effect of twelve hybrid GO nanocomposites (hGO NCs) (N₂-GO-HA, P-GO-HA, S-GO-HA, N₂-GO-SiO₂, P-GO-SiO₂, S-GO-SiO₂, N₂-GO-TiO₂, P-GO-TiO₂, S-GO-TiO₂, N₂-GO-Au, P-GO-Au and S-GO-Au) was assessed in caprine erythrocytes at 100, 50, 25, 10, and 0 μg/ml doses by hemolysis assay. In this study, it was observed that, significantly (P<0.01) highest (5.06±0.50%) and lowest (1.90±0.12%) hemolysis was occurred in caprine erythrocytes treated with S-GO-SiO₂ NC and P-GO-TiO₂ NC, respectively. Caprine erythrocytes were significantly (P<0.05) hemolysed by all twelve hGO NCs at 100 and 50 μg/ml doses as compared to 25, 10 and 0 μg/ml doses except S-GO-HA, N₂-GO-TiO₂, N₂-GO-Au, P-GO-Au and S-GO-Au where, only slight dose dependent hemolysis was observed. The present study is concluded that, S-GO-SiO₂ was highly hemolytic and P-GO-TiO₂ least hemolytic in caprine erythrocytes and however, all hGO NCs were cytocompatible at low doses (25 and 10 μg/ml) doses and hemolytic at high doses (100 and 50 μg/ml) in caprine erythrocytes.