This study aimed to assess the genetic relationship of Vietnamese gaurs from Central Highland and other gaurs from the Southeast Asia and India. The results showed that 15 polymorphic sites were found in 16S sequences between these gaurs. Vietnamese gaurs exhibited 15 identical SNPs with Cambodian gaur, while exposed 7 identical SNPs to Malayan gaur. The genetic distance analysis demonstrated that Vietnamese gaur showed the lowest distance to Bos frontalis (0.0086±0.0022) and Bos gaurus (0.0182±0.0054). Phylogenetic tree analysis indicated 3 groups of gaurs. Vietnamese gaur located in clade III which concluded Bos gaurus, Bos javanicus, Bos frontalis.