author(s)
Anderson Mathias Holtz, Ana Beatriz Mamedes Piffer, Priscila Stinguel de Azevedo, Julielson Oliveira Ataíde, Ronilda Lana Aguiar, Amanda Gonçalves Alves, Matheus de Paula Gomes, Patrícia Soares Furno Fontes, Bruna de Oliveira Magnani, Camila Groner Milbratz and Thiago Nieiro Cuzzuol

abstract
The two-spotted spider mite, Tetranychus urticae Koch, is one of the most important mite species in the world, this specie is related to attacking more than 250 crops of economic importance. This pest can occur year-round but grows best in warmer and drier times. This way, the objective of this work was to evaluate the acaricidal potential of the extract of the jatropha pie (Jatropha curcas L), in different concentrations, aiming for the pest management of T. urticae. The solutions were sprayed under sheets of Canavalia ensiformis, with the support of the Potter tower, consisting of direct application. In the indirect route of application, the leaf discs were immersed in the extract solution of the J. curcas, placed on a paper towel to dry, and subsequently, transferred to Petri dishes, with cotton moistened on the bottom and sides. Females of T. urticae were transferred to leaf discs. Ten replicates were used per treatment, evaluating the mortality as a function of post-spraying time. At all concentrations tested, the direct application treatment was superior to the indirect application, causing greater mortality of the two-spotted spider mite in laboratory conditions.