Munia Conservation Reserve (MCR), Nagpur is an important protected area lies on wildlife corridor from Pench Tiger Reserve to Tadoba Andhari Tiger Reserve with richest biodiversity. This study presents the initial diversity and distribution records of spider fauna in the Munia Conservation Reserve. In present study, 46 spider species identified, belonging to 42 genera and 18 families. The relative abundance was assessed, with Araneidae and Salticidae contributing significantly. The Shannon Wiener diversity index (H) is 1.978 and Evenness (E) is 0.684 for sample (N=18) of 42 genera and18 families with 46 species. The findings highlight the importance of Munia Conservation Reserve in supporting a rich spider fauna and contribute to the baseline understanding of spider diversity in the region and provide vital information for conservation and management efforts.