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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

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2018, Vol. 6, Issue 1
Bio-prevalence and molecular diagnosis of Mycobacterium avium Subsp paratuberculosis infection in small ruminant population of Ganderbal district of Kashmir valley

Adil Majid Bhat, HU Malik, SV Singh, Tufail Hussain, KK Chaubhey, MS Mir, Sabia Qureshi, ZA Kashoo, Showkat-ul-Nabi, Manzoor-ur-Rehman, Rather Waseem Yousuf and Syed Ishfaq Qadri

Present study first time determined bio-prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in sheep population of Ganderbal district of Kashmir valley. A total of 288 faecal samples (260 sheep and 28 goats) collected from 23 small ruminant farms were screened using a multi-stage simple radom sampling technique. A total of 40 faecal samples positive on microscopy were subjected to DNA isolation and IS900 PCR to confirm the presence of MAP. Bio-prevalence of animals shedding acid fast bacilli (AFB), indistinguishable to MAP was 32.9% (92). Block-wise bio-prevalence of AFB shedders in small ruminants was: Ganderbal (29.4%), Lar (35.2%), Wakura (32.5%) and Kangan (30.88%). Of the 40 faecal samples subjected to DNA isolation and amplification by IS900 PCR, 8 (20.0%) samples were positive for MAP. The study reported moderate bio-prevalence of MAP infection in small ruminant population of Ganderbal district of Kashmir valley.
Pages : 01-04 | 843 Views | 297 Downloads
How to cite this article:
Adil Majid Bhat, HU Malik, SV Singh, Tufail Hussain, KK Chaubhey, MS Mir, Sabia Qureshi, ZA Kashoo, Showkat-ul-Nabi, Manzoor-ur-Rehman, Rather Waseem Yousuf and Syed Ishfaq Qadri. Bio-prevalence and molecular diagnosis of Mycobacterium avium Subsp paratuberculosis infection in small ruminant population of Ganderbal district of Kashmir valley. 2018; 6(1): 01-04.
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