Histological and ultrastructural aspects of larval corpus allatum of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) treated with diflubenzuron and chromafenozide
Saleh TA, Ahmed KS, El-Bermawy SM, Ismail EH and Abdel-Gawad RM
The present investigation was undertaken to follow the two insect growth regulators (IGRs), diflubenzuronand chromafenozide, possible effects on the histology and ultrastructure aspects of corpus allatum (CA) of 6th instar larvae of Spodoptera littoralis. Therefore, the LC50 (3 ppm of diflubenzuron and 0.1 ppm of chromafenozide) were applied to the 4th larval instar. The CA of 6th larval instar treated with LC50 of diflubenzuron appeared with rounded shape and decreased size (265.625 µm width & 331.25 µm length) and the capsular fibrous sheath (3.380 µm) also reduced. Contradictory, cellular cortex was increased in size (71.875 µm), as well as glandular cell numbers were increased, and their nuclei were lost their spheroid shape. Disturbance in cytoplasmic organelles pushed glandular cell to switch off or to be inactive. Also, damage was pronounced in the CA of the LC50- chromafenozidetreated larvae. The present work investigates the high potency and efficacy of the two IGRs towards S. littoralis corpora allata.
Saleh TA, Ahmed KS, El-Bermawy SM, Ismail EH, Abdel-Gawad RM. Histological and ultrastructural aspects of larval corpus allatum of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) treated with diflubenzuron and chromafenozide. J Entomol Zool Stud 2018;6(3):864-872.