author(s)
Sudhanshu Pratap Singh, Ankesh Kumar, Prakrutik Prafulchandra Bhavsar, Mukesh Sahu, Praveen Kumar and Sushil Kumar

abstract
The present study was focused to study the effects of parenteral administrations of GnRH analogue-Buserelin acetate, Progesterone and Tolfenamic acid on biochemical parameters viz., serum glucose, serum cholesterol and serum total protein in repeat breeding animals. A total of 32 repeat-breeder crossbred cows were selected and divided in 4 equal groups (n=8). Group 1 was kept as positive control i.e. without any treatments, Group 2 animals were treated with Buserelin acetate, Group 3 animals were treated with Buserelin acetate + Progesterone and Group 4 animals were treated with inj. Buserelin acetate + Progesterone + Tolfenamic acid . Biochemical analysis was done by commercially available diagnostic kits. The mean blood glucose and serum protein concentration differed non- significantly without any changes in the normal range. The mean value of total serum cholesterol concentration on day 0 varies significantly (p<0.05) in group 2 & 4 compared to other groups. Moreover, on other days, cholesterol mean value was significantly (p<0.05) higher in group 4 as compared to other groups. Highest conception rate was observed in group 4 in which combinations of drugs were used.